". The performance of these two modes is not comparable to dedicated luminescence or timeresolved fluorescence instruments, or multimode readers such as the SpectraMax M5, Analyst HT or Analyst GT. The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays. Third this recommendation. The following document is intended. An integration time of 1 second was used for each reader. For more information, please visit us at Plate-Based Assays. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. An ATP standard curve spanning five decades was run using the ATPlite 1step Luminescence Assay System (PerkinElmer) on the SpectraMax iD5 reader. This. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. When you flip the LM1 plate over for checking Bottom Read, remove the Purple Plate adapter. The ultimate guide to microplate readers is an eBook that tells you everything you need to know about plate reader solutions from what a microplate reader is, to popular applications and technology, to GxP compliance and special tips and tricks to help maximize your. D 1 1. Wide range of applications. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. The plate is set up for reading and images are acquired according to specified parameters. plate type, read area and number of images per well, plus specify positive/negative wells. View online or download Molecular devices SpectraMax M5 User Manual. 2. In this. 04 to 3. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. 8. SpectraMax i3x reader measures absorbance, fluorescence, & luminescence with user-upgradeable application including western blot imaging, AlphaScreen, fast kinetics along with injectors plus additional detection modes. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. 40266 Email: [email protected] only) SpectraMax M-series Microplate readers. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . Winnersh Triangle. SpectraMax 250 User. Wokingham, Berkshire RG41 5TS United Kingdom. Supersedes 1. Choose from a three- (M3), four-. Please refer to the Obsolete Microplate Reader Models for more information. com 10. Wide range of applications The VersaMax reader covers a wide range of applications:Molecular Devices. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. 1. 18 x 14 x 8 ins. 2. The SpectraMax® M2 and M2e readers provide two detection modes in one platform, and offer the the added functionality of bottom-read fluorescence for cell-based. OPTICS Mirrored optics focus the light into the sample volume, and cutoff filters are used to. Simply unpack it, plug it in, and begin your experiments. The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. 95. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. Select Update Driver. Label: provides information. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 7 1. spectramax id5 multi-mode microplate reader, spectramax id5, spectramax paradigm, spectramax m5, spectramax m5 multi-mode microplate reader, high-throughput igg quantitation, mab igg titer, softmax pro, valita titer, valitatiter Created Date:1) Select the correct plate and the wells that you want to measure. 1. Figure 9. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. When the user applies a set temperature of 37°C, the top is heated to 37. MICROPLATE READER: Used, in good condition, working microplate reader. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. The chamber. SpectraMax M 系列多功能微孔板读板机（酶标仪）可测定紫外光 (UV) 和可见光 光吸收、荧光、发光、荧光偏振、时间分辨荧光 (TRF) 和均相时间分辨荧光 (HTRF) 。. optical beams than older wide-beam plate readers, are much more vulnerable to spurious readings due to dust. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. No additional manuals are available. Choose from a three- (M3), four- (M4), or Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Gemini XPS. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Cytation 5 combines automated microscopy and conventional microplate detection in a configurable, upgradable platform. Representative data from the SpectraMax i3x Multi-Mode Microplate Reader for both MycoAlert and MycoAlert PLUS assays are shown in Figure 3. Molecular Devices SpectraMax M5 Plate Reader available for sale at The Lab World Group. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). A solution for low volume applications. Brand: Molecular Devices | Category: Laboratory Equipment | Size: 0. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. . Bioz Stars score: 86/100, based on 1 PubMed citations. Microplate Reader Shelf. The Spectr. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and. The plate was incubated at room temperature for one hour. book Page 21 Wednesday, July 7, 2010 2:09 PM. There is currently no charge for using this plate reader. For a higher . Bookings are. The dedicated luminescence PMT has extremely low background counts and superior performance in the blue wavelengths. PERKIN ELMER Wallac Victor 2V Microplate Reader. Weighs about - 23 lbs. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Review date: 07 Nov 2017 | SpectraMax® M5 Multi-Mode Microplate Reader. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. For problems with SpectraMax Multi-Mode Microplate Readers that are not listed here, in the U. SpectraMax® M5 Multi-Mode Microplate Re. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Consider these features as well as all of the intended applications when choosing an appropriate luminescence reader for the lab. Page 1 of 8 . SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. the purple (SpectraMax M5 or M5e reader) or black (FlexStation 3 reader) plate adapter in the plate carriage prior to. Data analysis Standard curves were plotted using the quadratic curve fit in SoftMax Pro Software, and the concentrations of cell lysates were calculated from the standard curves by the software. The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Considerations for readers include the measurement wavelength range, microwell plate capacity, throughput, detection methods, and a filter vs monochromator-based optical system. Instructions. 4. 标准功. Self Service Available. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays’ HTRF (Homogeneous Time-Resolved Fluorescence) technology. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). Our absorbance plate readers feature our PathCheck Sensor technology and. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. We would like to show you a description here but the site won’t allow us. After incubation of the cells with compounds, add 100 µL (for 96-well plates) or 25 µL (for 384-well plates) of Dye Solution per test well. Figure 10: The Plate section Template Editor, showing the series set up. 40266 Email: drugdiscoverytech@lifetech. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. Detection modalities include absorbance (UV-Vis), fluorescence intensity, fluorescence polarization, time-resolved fluorescence and luminescence. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. Model: SpectraMax M5. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. What plates do you recommend for SpectraMax Plate Readers? Luminescence: Solid white plates for top reads and white plates with clear bottoms for bottom reads. Shop all used microplate readers and parts from a variety of manufacturers. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. After the plate is read, data will appear in the plate. 010 OD 384-Well Plate,. SpectraMax Plus, Plus384, M2, M2e, M5e or M5 readers). Set up a plate section for a second emission scan as in Step C above, except for the added Emission Cutoff Filter. Figure 1. Human macrophages choreograph tissue development. 0200-0729. 40266 Email: drugdiscoverytech@lifetech. The Cuvette Chamber (SpectraMax Plus 384 only) Figure 1. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. Spectramax M5. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. edu, 814-863-3503. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. When the plate was read on the SpectraMax M5 microplate reader, an enzyme concentration range of 0. Validation test plates for Abs, FI, Lum optical performance. The Molecular Devices SpectraMAX absorbance microplate readers mimic a dual beam spectrophotometer with a patented multichannel optical design. In the lower half of the window, left side, there is a box labeled reader. 9000-0756. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. Wide range of applications. ** Please reference item #181108-12454-08 when. Description 1. Which SpectraMax M Series reader do you need? SpectraMax M Series Multi-Mode Microplate Readers 5 Ordering information VWR cat. The SpectraMax® Quant™ dsDNA Assay Kits are designed for fluorescence-based dsDNA quantitation across a broad range of concentrations. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader Specifications. Here the delta mP was 336, and Z’ factor was 0. Temperature Regulation. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading STAKMAX. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. 7°C (gradient heating design). 25 fmol/well, 384-well plate)−top Fluorescein 4 pM (0. Experimental details and customization of the group table. Equipped with scanning, shaking and kinetics modules. TNF standard curve. 01) + 0. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Results. Information on how to reserve the plate readerThe GloMax® Explorer is a high-performance multimode plate reader that allows you to get up and running quickly, generating the data you need from your experiments. Supported Plates and Cuvettes. Do NOT use the front panel Ref button in fluorescence mode cuvette reads. FlexStation II 384. Which SpectraMax Multi-Mode Microplate Reader do you need? Detection Modes Absorbance Fluorescence Intensity Luminescence Time-Resolved Fluorescence Fluorescence Polarization HTRF Upgrade Options Plate Formats 6-, 12-, 24-, 48-, 96-, 384-Well Microplates Certification and Validation IMAP Validation (TR-FRET only) (TR-FRET. Parts Only. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. 312 gain calibration failed. 1) Select the correct plate and the wells that you want to measure. " SpectraMax M5 micro plate reader is a multi mode plate reader (FLU, LUMN . The Infinite and Spark multimode microplate readers are modular and upgradeable. Microplate Readers Fisher Scientific AccuSkan FC Microplate Reader – 2020. While they play an important role in generating data from a wide range of. However, when a specific probe is not available, or it causes off-target effects on the. The Molecular Devices SpectraMax M5E is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The SpectraMax® iD3 Multi-Mode Microplate Reader and the SpectraMax® iD5 Multi-Mode Microplate Reader from Molecular Devices® have a touch screen interface that provides integrated instrument control, data display, and the ability to export results over your network for statistical data analysis. Description. The SpectraMax® iD3 Multi Mode Microplate . The following document is. A wide range of assays can be performed using a microplate reader including ELISAs, protein cell growth assays, nucleic acid and protein quantification, enzyme activity measurement, immunoassays, and screening of drug compounds and targets, among. Molecular Devices announced today the launch of the new SpectraMax ® iD5 Multi-Mode Microplate Reader, a feature-rich platform that expands the boundaries of scientific research. The SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. Pathcheck Pathlength Measurement Technology. BMG Nephelostar Laser-Based Microplate Nephelometer Microplate Reader. • Tel. measure the depth (optical pathlength) of samples with no temperature. Tecan Infinite M1000 Microplate Reader w/ Injectors $10,000. youusehighprofile(6-well,12-well,24-well,or48-well)plates,youmustremovethe plateadapterfromtheplatedrawer. 筛选时，SpectraMax M5多功能微孔板读板 机可作为其非常理想的、可靠的检测平台。 SpectraMax M5是一种基于光栅型单色器 的多功能微孔板读板机，允许使用者针对不 同的荧光染料分子随意选择不同的检测波 长，而无需额外再选购滤光片配件。这篇应FlexStation® 3 Multi-Mode Microplate Reader. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. The chamber. Figure 2-23: Bottle holder To install the bottle holder: 1. used. Using an SpectraMax Microplate Reader for DNA absorbance test, there is one problem that the A260/A280 is always wrong, sometimes half plate wrong and sometime, several lanes. SpectraMax Glo Steady-Luc Reporter Assay Kit workflow. If it is something different, click on the arrow to bring up a list. SpectraMax M3, M4, M5, and M5 e. 0 . The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . Using the. The M5 can read volumes down to 2uL with the. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. SoftMax Pro Software can collect data from one or more microplates or cuvettes and store it in a single data file, using the same or different instrument settings for different microplates or cuvettes. All SpectraMax® M series multi-mode microplate readers measure UV and visible absorbance, and fluorescence intensity measurements on microplates and cuvettes as standard. spr SpectraMax iD3 SpectraTest ABS2 v1. From the Windows Start menu, go to All programs > Molecular Devices > SoftMax Pro x. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. Copia stocks a full line of used microplate readers. Molecular Devices SpectraMax M2e Multi-Mode Microplate Reader w/ SoftMax Pro. 2. 2. SpectraMax Spectrofluorometers. With optimized reagents and the industry-leading. M5 E -HTRF Readers. Thermo Scientific Microplate Reader Verification Plates Cat# 24072800. The multi-mode. D 1. EarlyTox Caspase-3/7 R110 Assay. Reader Documentation Version No. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 0112-0115 F . The BD plate dimensions are 5. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 1. Italic and boldface type are used for emphasis. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. The SpectraMax® ABS Microplate Reader bridges the gap between the affordability of filter-based readers and the . For more information, please visit us at microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. Label-free detection. Uncontrolled Copy for Training and Reference Purposes Only . The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. 1321N1, a human astrocytoma cell line, expresses. Detection modalities. individual cuvettes (using SpectraMax Plus, Plus384, M2, M2e, M5e or M5). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. The Perkin Elmer Wallac 1420 VICTOR2™ is a high performing microplate reader built for multi-tasking functions. it is already turned on and the screen should look like this with “SpectraMax M5” showing as connected in the top left: 7. Put the plate in the SPECTRAmax PLUS drawer, then click the Read but-ton in the Plate section’s Status Bar. use two scanning monochromators so the user can determine optimal excitation and emission settings, resulting in assay performance similar to that of dedicated single-mode readers. Optics. Setup Guide on Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. To set up how to read the plate you can load an existing protocol from the protocol manager/protocol library or use the plate setup helper shown at the cursor below: 8. To assess the sensitivity of each microplate reader, a plate was set up with 32 wellsThe SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. HTRF-compatible SpectraMax Multi-Mode Readers. 0% ± 0. For more information, please visit us at Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. Turn the reader on and secure the USB cable. These robust readers have been placed in labs from Antarctica to. Choose the desired plate type, using the upper dropdown menu to choose plate format (96 orMolecular Devices Spectramax M5 Plate Reader is available from The Lab World Group. SpectraMax M5 Plate Reader (Molecular Devices) If you have trouble accessing this page and need to request an alternate format, contact u@osu. IMAP phosphodiesterase assays on SpectraMax Multi-Mode Microplate Readers. : 8 Oct 12 Page 7 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. i3x. For more sample throughput, the system canThe SpectraMax M5 plate reader which used to be in the Millennium Science Complex, W-124A is now located in the Proteomics and MS Core Facility, in 3 Althouse. settings listed below for the SpectraMax iD5 reader can be used for any SpectraMax iD5 reader. The SpectraMax M5 Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. For example, microplates containing different samp les can be read using the same orThe Molecular Devices SpectraMax M2 Microplate Reader is a multidetection microplate reader with dualmonochromators, dual-mode cuvette ports, and top-reading capability. The plate was vigorously mixed and incubated for 4 min at room temperature in the dark. Figure 1. 5 units/mL yielded a delta mP of 303, with a Z’ factor of 0. 7°C (gradient heating design). 0 . For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?The SpectraMax M5 utilizes a single monochromator design for UV/VIS Absorbance, allowing the user to maximize sensitivity and tunability, and also uses patented PathCheck Pathlength Measurement Technology: the only temperature independent pathlength correction for microplates. 0 . For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. Catalog Number: M5. After the plate is read, data will appear in the plate section: 11. Microplate Readers. Designed to provide the simplicity, flexibility and power required for advanced data analysis. The SpectraMax M3 Microplate Reader is supplied with SoftMax Pro Data Acquisition and Analysis Software, Molecular Devices' industry leading all-in-one software. 000000 out of 5. M5 and M5e Multi-Mode Microplate Readers User Guide SpectraMax M3, M4, M5. The plate reader. Or, with the reader specifications noted from the Instrument Description section of your reader's manual, as well as those for your PC, you can calculate needs more closely. Wide range of applications. 000). columns 1, 3, 5, 7, 9 and 11 were seeded with 200,000 mock-transfected cells per well. 0. Fluorescence intensity was measured using a. It also integrates with the. Page 1 of 8 . . 1. Multimode Reader Comparison Chart. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. 1. There is currently no charge for using this plate reader. At NanoRacks, the challenge for the engineers will be to modify the plate reader in order for the unit to operate comfortably in the zero-gravity environment of the space station. 72 MB. Our absorbance plate readers feature our PathCheck Sensor. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. Tailored to your different needs, these assay kits are configured and optimized for Molecular Devices SpectraMax® microplate readers, with preconfigured protocols provided in SoftMax® Pro Software for. Please contact Tatiana to reserve the plate reader time: tnl1@psu. Microplate Reader, Multimode. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Shop now. Choose monochromator-based optics for flexibility, filter-based optics for sensitivity, or both…BioTek’s patented Hybrid Technology™ offers high-performance and applications versatility in a modular platform to expand as your laboratory’s. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI) and have optical performance comparable to a top-of-the-line dedicated spectrofluorometer or. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Five preferred detection modes. SpectraMax M5 reader SpectraMax M5e. Following treatment the samples were assayed for protease activity in triplicate using the Pierce Fluorescent Protease Assay Kit (Thermo Scientific, Waltham USA),. SoftMax Pro. Description 1. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Qualify the absorbance performance of SpectraMax® iD3, iD5, i3, i3x, M2, M2e, M3, M4, M5, M5e, Plus 384, ABS, ABS Plus, and FlexStation 3 readers. Description. The drawing shows a standard microplate of 15 mm height covered with a lid. FlexStation II. The SpectraDrop Micro-Volume microplate offers the ability to use as little as 2 µL samples with 24 or 64-well plates. SpectraMax® M2 Multi-Mode Microplate Reader MLDVM2 SpectraMax® M2e Multi-Mode Microplate Reader (top and bottom reading) MLDVM2E SpectraMax® M3 Multi-Mode Microplate Reader MLDVM3. Test up to 3. 40266 Email: drugdiscoverytech@lifetech. Plate Shaking Linear, Orbital Slow, and Orbital Fast. Absorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. Fluorescence Microplate Reader Comparison Chart. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Developed with Promega reagents to provide a. The standard curve was plotted using a log-log curve fit in the SoftMax® Pro Software (r2 = 1. The microscopy module offers up to 60x magnification in fluorescence, brightfield, high contrast brightfield, color brightfield, and phase contrast to address many applications and workflows. Molecular Devices Spectramax M5 Microplate Reader. 0 OD for earlier models. 단일 튜브 디스펜싱에서 전체 96 및 384 well 플레이트세척까지 간소화된 Microplate 세척입니다. 00. Molecular Devices SpectraMax Plus 384. Your applications, your modes, your choice. View the Molecular Devices website. Setup for Z’-LYTE® Assay on SpectraMax® M5/M5e Microplate Reader with SoftMax® Pro 6 Software. We would like to show you a description here but the site won’t allow us. An assay plate containing a 15-point standard curve was read on the ® M5 Microplate reader. Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. This reader's dual monochromators for. 0 out of 5 $ 16,499. IMAP assays are based on binding of. Our multi-mode plate readers provide great flexibility and include absorbance, fluorescence and luminescence with configurable options for fluorescence polarization (FP), time. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. Reader Documentation Version No. Ensure data accuracy of your reader. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 0 out of 5 $ 3,799. 000 OD ± 1. Locate the User Guide on your SoftMax Pro computer for version 6 and version 7. com 10. Rating: 5. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. In the M2, M5 and Plus 384, the light. SpectraMax M5 Plate Reader SpectraMax M5 is a multi-detection microplate reader with dual monochromators, a triple-mode cuvette port, and 6-384 well microplate reading capability. Extended linear dynamic range. Your applications, your modes, your choice. 2. M3-M5e_manual. spr * SpectraMax i3(x) SpectraTest ABS2 v1. SpectraMax M5. Read More. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. 025 fmol/well, 384-well plate) Quad monochromator: Fluorescein 2. TR-FRET assay principle. Third this recommendation. 40266 Email: drugdiscoverytech@lifetech. By offering two new configurations of multi-mode readers that deliver increased capabilities over the M2/M2e Dual-Mode Readers at a more affordable price than the top-of-the-line M5/M5e Multi-Mode Readers, MDS Analytical Technologies can now meet an even greater combination of detection, application, and budgetary needs. The instrument settings were identical to those used in Example 1 above. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Endpoint, kinetic, spectrum and area-well scanning read types and the PathCheck®. Abs).